Recombinant humaan MRPL28-eiwit

MRPL28, also as known as 39S ribosomal protein L28, mitochondrial, is a mitochondrial protein that belongs to the ribosomal protein L28P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL28 potentially represents an important therapeutic reagent for HLA-A24 (A24) patients as this antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype. This protein is found in a variety of normal tissues including spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. Recombinant human MRPL28 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

39S ribosomal protein L28 mitochondrial, 39S ribosomal protein L28, mitochondrial, MAAT1, p15

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.8 kDa (222aa) confirmed by MALDI-TOF

MRPL28, 39S ribosomal protein L28 mitochondrial, 39S ribosomal protein L28, mitochondrial, MAAT1, p15, ATGP1802-10 µg, ATGP1802-20 µg, ATGP1802-50 µg, ATGP1802-100 µg, ATGP1802-250 µg, ATGP1802-500 µg, ATGP1802-1 mg, ATGP1802-10, ATGP1802-20, ATGP1802-50, ATGP1802-100, ATGP1802-250, ATGP1802-500, ATGP1802-1

Koc EC, et al. (2001) J Biol Chem 276 (47) : 43958-69.; ; Kawakami, Y., et al. (2000) J.Immunother. 23: 17-27.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006419

Q13084

Human

MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ