Recombinant humaan MRPL48-eiwit

Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Recombinant human MRPL48 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Mitochondrial ribosomal protein L48, CGI-118, HSPC290, L48MT, MRP-L48

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.1 kDa (207aa)

MRPL48, Mitochondrial ribosomal protein L48, CGI-118, HSPC290, L48MT, MRP-L48, ATGP2839-10 µg, ATGP2839-20 µg, ATGP2839-50 µg, ATGP2839-100 µg, ATGP2839-250 µg, ATGP2839-500 µg, ATGP2839-1 mg, ATGP2839-10, ATGP2839-20, ATGP2839-50, ATGP2839-100, ATGP2839-250, ATGP2839-500, ATGP2839-1

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; ; Lai C.-H., et al. (2000) Genome Res. 10:703-713

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_057139

Q96GC5

Human

MGSSHHHHHH SSGLVPRGSH MGSSGEKPIY SVGGILLSIS RPYKTKPTHG IGKYKHLIKA EEPKKKKGKV EVRAINLGTD YEYGVLNIHL TAYDMTLAES YAQYVHNLCN SLSIKVEESY AMPTKTIEVL QLQDQGSKML LDSVLTTHER VVQISGLSAT FAEIFLEIIQ SSLPEGVRLS VKEHTEEDFK GRFKARPELE ELLAKLK