Recombinant humaan MRPS28-eiwit

Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. Recombinant human MRPS28 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

28S ribosomal protein S28 mitochondrial, 28S ribosomal protein S28, mitochondrial, HSPC007, MRP-S28, MRP-S35, MRPS35

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.5 kDa (193aa) confirmed by MALDI-TOF

MRPS28, 28S ribosomal protein S28 mitochondrial, 28S ribosomal protein S28, mitochondrial, HSPC007, MRP-S28, MRP-S35, MRPS35, ATGP2936-10 µg, ATGP2936-20 µg, ATGP2936-50 µg, ATGP2936-100 µg, ATGP2936-250 µg, ATGP2936-500 µg, ATGP2936-1 mg, ATGP2936-10, ATGP2936-20, ATGP2936-50, ATGP2936-100, ATGP2936-250, ATGP2936-500, ATGP2936-1

Zhang Q.-H., et al. (2000) Genome Res. 10:1546-1560.; ; Kenmochi N., et al. (2001) Genomics 77:65-70;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid In. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Epigenetics and Nuclear Signaling

NP_054737

Q9Y2Q9

Human

GSPKNVE SFASMLRHSP LTQMGPAKDK LVIGRIFHIV ENDLYIDFGG KFHCVCRRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT DTTVLEANAV LLGIQESKDS RSKEEHHEK