Recombinant humaan MRRF-eiwit

MRRF binds to the large ribosomal subunit in the cleft that contains the peptidyl transferase center. This protein is responsible for the release of ribosome from messenger RNA at the termination of protein biosynthesis. Also, it may increase the efficiency of translation by recycling ribosome from one round of translation to another. Recombinant human MRRF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.1 kDa (228aa) confirmed by MALDI-TOF

MRRF, Ribosome-recycling factor mitochondrial isoform 1, Ribosome-recycling factor, mitochondrial isoform 1, MRFF, MTRRF, RRF, ATGP1826-10 µg, ATGP1826-20 µg, ATGP1826-50 µg, ATGP1826-100 µg, ATGP1826-250 µg, ATGP1826-500 µg, ATGP1826-1 mg, ATGP1826-10, ATGP1826-20, ATGP1826-50, ATGP1826-100, ATGP1826-250, ATGP1826-500, ATGP1826-1

Hirokawa G., et al. (2008) Nucleic Acids Res. 36 (21) :6676-87; ; Pai RD., et al. (2008 J Mol Biol. 376 (5) :1334-47.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_620132

Q96E11

Human

ATKKAKAKG KGQSQTRVNI NAALVEDIIN LEEVNEEMKS VIEALKDNFN KTLNIRTSPG SLDKIAVVTA DGKLALNQIS QISMKSPQLI LVNMASFPEC TAAAIKAIRE SGMNLNPEVE GTLIRVPIPQ VTREHREMLV KLAKQNTNKA KDSLRKVRTN SMNKLKKSKD TVSEDTIRLI EKQISQMADD TVAELDRHLA VKTKELLG