Recombinant humaan MSRA-eiwit

MSRA (methionine sulfoxide reductase A) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R) -S-oxide and thioredoxin. Recombinant human MSRA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.2 kDa (237aa) confirmed by MALDI-TOF

MSRA, methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2, ATGP1569-10 µg, ATGP1569-20 µg, ATGP1569-50 µg, ATGP1569-100 µg, ATGP1569-250 µg, ATGP1569-500 µg, ATGP1569-1 mg, ATGP1569-10, ATGP1569-20, ATGP1569-50, ATGP1569-100, ATGP1569-250, ATGP1569-500, ATGP1569-1

Boschi-Muller S, et al. (2005) Biochim. Biophys. Acta. 1703 (2) : 231-8. ; ; Hansel A., et al. (2005) Biochim. Biophys. Acta 1703:239-247.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Redox Proteins

NP_036463

Q9UJ68

Human

MGSSHHHHHH SSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGA ERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMR QGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNG YCGLGGTGVS CPVGIKK