Recombinant humaan MT3-eiwit

Metallothionein 3, also known as MT3, binds heavy metals. This protein contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. This protein inhibits survival and neurite formation of cortical neurons in vitro. Recombinant human MT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Metallothionein 3, Metallothionein 3, GIF, GIFB, GRIF, ZnMT3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

9.3 kDa (91aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

MT3, Metallothionein 3, Metallothionein 3, GIF, GIFB, GRIF, ZnMT3, ATGP2535-10 µg, ATGP2535-20 µg, ATGP2535-50 µg, ATGP2535-100 µg, ATGP2535-250 µg, ATGP2535-500 µg, ATGP2535-1 mg, ATGP2535-10, ATGP2535-20, ATGP2535-50, ATGP2535-100, ATGP2535-250, ATGP2535-500, ATGP2535-1

Uchida Y., et al. (1991) Neuron. 7:337-347; ; Palmiter R.D., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:6333-6337;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Metabolism

NP_005945.1

P25713

Human

MDPETCP CPSGGSCTCA DSCKCEGCKC TSCKKSCCSC CPAECEKCAK DCVCKGGEAA EAEAEKCSCC Q