Recombinant humaan MTHFD2-eiwit

MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.2 kDa (344aa) confirmed by MALDI-TOF

MTHFD2, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NMDMC, ATGP2972-10 µg, ATGP2972-20 µg, ATGP2972-50 µg, ATGP2972-100 µg, ATGP2972-250 µg, ATGP2972-500 µg, ATGP2972-1 mg, ATGP2972-10, ATGP2972-20, ATGP2972-50, ATGP2972-100, ATGP2972-250, ATGP2972-500, ATGP2972-1

Watkins D., et al. (2011) Genet. 48 (9) : 590-2.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Metabolism

NP_006627.2

P13995

Human

LAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN