Recombinant humaan MTHFS-eiwit

MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5, 10-methenyltetrahydrofolate (MTHF) . MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD) . Recombinant human MTHFS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

5-formyltetrahydrofolate cyclo-ligase, Methenyl-THF synthetase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.4 kDa (223aa) confirmed by MALDI-TOF

MTHFS, 5-formyltetrahydrofolate cyclo-ligase, Methenyl-THF synthetase, ATGP0976-10 µg, ATGP0976-20 µg, ATGP0976-50 µg, ATGP0976-100 µg, ATGP0976-250 µg, ATGP0976-500 µg, ATGP0976-1 mg, ATGP0976-10, ATGP0976-20, ATGP0976-50, ATGP0976-100, ATGP0976-250, ATGP0976-500, ATGP0976-1

Field, M.S., et al. (2006) J. Biol. Chem. 281: 4215-4221.; ; Lim, u., et al. (2007) Blood 109: 3050-3059.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl

Metabolism

NP_006432

P49914

Human

AAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA