Recombinant humaan Musashi-2-eiwit

MSI2 is a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. Recombinant human MSI2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Musashi homolog 2, MSI2H

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.6 kDa (351aa) confirmed by MALDI-TOF

Musashi2, ATGP2179-10 µg, ATGP2179-20 µg, ATGP2179-50 µg, ATGP2179-100 µg, ATGP2179-250 µg, ATGP2179-500 µg, ATGP2179-1 mg, ATGP2179-10, ATGP2179-20, ATGP2179-50, ATGP2179-100, ATGP2179-250, ATGP2179-500, ATGP2179-1

Daub H., et al. (2008) Mol. Cell. 31:438-448; ; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_620412

Q96DH6

Human

MEANGSQ GTSGSANDSQ HDPGKMFIGG LSWQTSPDSL RDYFSKFGEI RECMVMRDPT TKRSRGFGFV TFADPASVDK VLGQPHHELD SKTIDPKVAF PRRAQPKMVT RTKKIFVGGL SANTVVEDVK QYFEQFGKVE DAMLMFDKTT NRHRGFGFVT FENEDVVEKV CEIHFHEINN KMVECKKAQP KEVMFPPGTR GRARGLPYTM DAFMLGMGML GYPNFVATYG RGYPGFAPSY GYQFPGFPAA AYGPVAAAAV AAARGSGSNP ARPGGFPGAN SPGPVADLYG PASQDSGVGN YISAASPQPG SGFGHGIAGP LIATAFTNGY H