Recombinant humaan Muscle Fosfofructokinase/PFKM-eiwit

PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1, 6-bisphosphate (through PFK-1), fructose 2, 6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

6-Phosphofructokinase muscle type, GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase.

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

87.3 kDa (800aa) confirmed by MALDI-TOF

Muscle Phosphofructokinase, PFKM, 6-Phosphofructokinase muscle type, GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase., ATGP0536-10 µg, ATGP0536-20 µg, ATGP0536-50 µg, ATGP0536-100 µg, ATGP0536-250 µg, ATGP0536-500 µg, ATGP0536-1 mg, ATGP0536-10, ATGP0536-20, ATGP0536-50, ATGP0536-100, ATGP0536-250, ATGP0536-500, ATGP0536-1

Martinez-Costa OH., et al. (2007) FEBS Lett. 581 (16) :3033-8. ; ; Kim JW., et al. (2004) Mol Cell Biol. 24 (13) :5923-36.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 0.2 M NaCl, and 20% glycerol

Cancer

NP_000280

P08237

Human

MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV