Recombinant humaan MUTED-eiwit

Muted homolog, also known as MuTED, is involved in the development of lysosome related organelles, such as melanosomes and platelet dense granules. MuTED interacts with pallidin, dystrobrevin binding protein 1 and CNO/cappuccino. It is part of the biogenesis of lysosome related organelles complex 1 (BLOC1) . Also, MuTED is ubiquitously expressed with higher levels in brain, bone marrow, kidney, and liver and lower levels in skeletal muscle. Recombinant human MuTED protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Muted homolog, Mu

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24 kDa (210aa) confirmed by MALDI-TOF

MUTED, Muted homolog, Mu, ATGP1631-10 µg, ATGP1631-20 µg, ATGP1631-50 µg, ATGP1631-100 µg, ATGP1631-250 µg, ATGP1631-500 µg, ATGP1631-1 mg, ATGP1631-10, ATGP1631-20, ATGP1631-50, ATGP1631-100, ATGP1631-250, ATGP1631-500, ATGP1631-1

Ciciotte S L., et al. (2003) Blood. 101: 4402-4407; ; Starcevic M., et al. (2004) J Biol Chem. 279: 28393-28401.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol,2mM DTT, 0.1mM PMSF, 1mM EDTA

Signal Transduction

NP_958437

Q8TDH9

Human

MGSSHHHHHH SSGLVPRGSH MGSMSGGGTE TPVGCEAAPG GGSKKRDSLG TAGSAHLIIK DLGEIHSRLL DHRPVIQGET RYFVKEFEEK RGLREMRVLE NLKNMIHETN EHTLPKCRDT MRDSLSQVLQ RLQAANDSVC RLQQREQERK KIHSDHLVAS EKQHMLQWDN FMKEQPNKRA EVDEEHRKAM ERLKEQYAEM EKDLAKFSTF