Recombinant humaan MVD-eiwit

MVD, also known as diphosphomevalonate decarboxylase, catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Recombinant human MVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Diphosphomevalonate decarboxylase, MPD

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

45.6 kDa (420aa) confirmed by MALDI-TOF

MVD, diphosphomevalonate decarboxylase, MPD, ATGP1354-10 µg, ATGP1354-20 µg, ATGP1354-50 µg, ATGP1354-100 µg, ATGP1354-250 µg, ATGP1354-500 µg, ATGP1354-1 mg, ATGP1354-10, ATGP1354-20, ATGP1354-50, ATGP1354-100, ATGP1354-250, ATGP1354-500, ATGP1354-1

Toth M.J., et al. (1996) J. Biol. Chem. 271:7895-7898; ; Molina H., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 104:2199-2204

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Metabolism

NP_002452

P53602

Human

MGSSHHHHHH SSGLVPRGSH MASEKPLAAV TCTAPVNIAV IKYWGKRDEE LVLPINSSLS VTLHQDQLKT TTTAVISKDF TEDRIWLNGR EEDVGQPRLQ ACLREIRCLA RKRRNSRDGD PLPSSLSCKV HVASVNNFPT AAGLASSAAG YACLAYTLAR VYGVESDLSE VARRGSGSAC RSLYGGFVEW QMGEQADGKD SIARQVAPES HWPELRVLIL VVSAEKKLTG STVGMRASVE TSPLLRFRAE SVVPARMAEM ARCIRERDFP SFAQLTMKDS NQFHATCLDT FPPISYLNAI SWRIIHLVHR FNAHHGDTKV AYTFDAGPNA VIFTLDDTVA EFVAAVWHGF PPGSNGDTFL KGLQVRPAPL SAELQAALAM EPTPGGVKYI IVTQVGPGPQ ILDDPCAHLL GPDGLPKPAA