Recombinant humaan MYL12A-eiwit

MYL12A, also known as myosin regulatory light chain 12A, plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. This protein implicated in cytokinesis, receptor capping, and cell locomotion. Recombinant human MYL12A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Myosin regulatory light chain 12A, MLCB, MRCL3, MRLC3, MYL2B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.4 kDa (195aa) confirmed by MALDI-TOF

MYL12A, ATGP1163-10 µg, ATGP1163-20 µg, ATGP1163-50 µg, ATGP1163-100 µg, ATGP1163-250 µg, ATGP1163-500 µg, ATGP1163-1 mg, ATGP1163-10, ATGP1163-20, ATGP1163-50, ATGP1163-100, ATGP1163-250, ATGP1163-500, ATGP1163-1

Grant J.W., et al. (1990) Nucleic Acids Res. 18:5892-5892; ; Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Cytoskeleton

NP_006462

P19105

Human

MSSKRT KTKTKKRPQR ATSNVFAMFD QSQIQEFKEA FNMIDQNRDG FIDKEDLHDM LASLGKNPTD EYLDAMMNEA PGPINFTMFL TMFGEKLNGT DPEDVIRNAF ACFDEEATGT IQEDYLRELL TTMGDRFTDE EVDELYREAP IDKKGNFNYI EFTRILKHGA KDKDD