Recombinant humaan MYL5-eiwit

Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myosin regulatory light chain 5 isoform 2, Myosin regulatory light chain 5, isoform 2, MYL 5, MyLC 2, MyLC2, Myosin light chain 5, Myosin light chain 5 regulatory, Myosin light polypeptide 5, Myosin light polypeptide 5 regulatory, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.4 kDa (156aa) confirmed by MALDI-TOF

MYL5, Myosin regulatory light chain 5 isoform 2, Myosin regulatory light chain 5, isoform 2, MYL 5, MyLC 2, MyLC2, Myosin light chain 5, Myosin light chain 5 regulatory, Myosin light polypeptide 5, Myosin light polypeptide 5 regulatory, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, ATGP1188-10 µg, ATGP1188-20 µg, ATGP1188-50 µg, ATGP1188-100 µg, ATGP1188-250 µg, ATGP1188-500 µg, ATGP1188-1 mg, ATGP1188-10, ATGP1188-20, ATGP1188-50, ATGP1188-100, ATGP1188-250, ATGP1188-500, ATGP1188-1

Collins C., et al. (1993) Hum Mol Genet. 1:727-733.; ; Lowey S., et al. (1993) Nature. 365:454-456.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Cytoskeleton

AAH40050.1

Q02045

Human

MDQNRD GFIDKEDLKD TYASLGKTNV KDDELDAMLK EASGPINFTM FLNLFGEKLS GTDAEETILN AFKMLDPDGK GKINKEYIKR LLMSQADKMT AEEVDQMFQF ASIDVAGNLD YKALSYVITH GEEKEE