Recombinant humaan MYL6B-eiwit

MYL6B is a heavy chain regulator found in smooth muscle and non-muscle Myosin complexes. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain by Myosin light chain kinase. MYL6B does not bind calcium during contraction. It is primarily found in a hexamer consisting of four light chains and two heavy chains. It most commonly interacts with Myosin Va, an Actin based motor that can move in large steps. MYL6B is expressed in most tissues with neurons and smooth muscle tissue having the highest expression. Recombinant human MYL6B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myosin light chain 6B, MLC1SA

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.2 kDa (231aa) confirmed by MALDI-TOF

MYL6B, Myosin light chain 6B, MLC1SA, ATGP1206-10 µg, ATGP1206-20 µg, ATGP1206-50 µg, ATGP1206-100 µg, ATGP1206-250 µg, ATGP1206-500 µg, ATGP1206-1 mg, ATGP1206-10, ATGP1206-20, ATGP1206-50, ATGP1206-100, ATGP1206-250, ATGP1206-500, ATGP1206-1

Mitsui T., et al. (1992) J Biol Chem. 267 (23) :16727-35.; ; Takeya K., et al. (2008) Am J Physiol Renal Physiol. 294 (6) :1487-92.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT

Cytoskeleton

NP_002466

P14649

Human

MGSSHHHHHH SSGLVPRGSH MGSMPPKKDV PVKKPAGPSI SKPAAKPAAA GAPPAKTKAE PAVPQAPQKT QEPPVDLSKV VIEFNKDQLE EFKEAFELFD RVGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEL KSRRVDFETF LPMLQAVAKN RGQGTYEDYL EGFRVFDKEG NGKVMGAELR HVLTTLGEKM TEEEVETVLA GHEDSNGCIN YEAFLKHILS V