Recombinant humaan MYL9-eiwit

MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant human MYL9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myosin light chain 9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MLC2, MYRL2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.9 kDa (192aa) confirmed by MALDI-TOF

MYRL2, MYRL 2, Myosin RLC, Myosin regulatory light polypeptide9, Myosin regulatory light polypeptide 9, Myosin regulatory light chain MRLC1, Myosin regulatory light chain 9, Myosin regulatory light chain 2 smooth muscle isoform, Myosin light chain 9, MYL9, MRLC 1, MLC-2C, MLC2, MLC 2, MGC 3505, M RLC 1, LC20, LC 20, 20kDa myosin light chain, 20 kDa myosin light chain, ATGP0923-10 µg, ATGP0923-20 µg, ATGP0923-50 µg, ATGP0923-100 µg, ATGP0923-250 µg, ATGP0923-500 µg, ATGP0923-1 mg, ATGP0923-010, ATGP0923-020, ATGP0923-050, ATGP0923-100, ATGP0923-250, ATGP0923-500, ATGP0923-01M

Gilles L., et al. (2009) Blood. 114 (19) :4221-32.; ; Medjkane S., et al. (2009) Nat Cell Biol. 11 (3) :257-68.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Cytoskeleton

NP_006088

P24844

Human

MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD