Recombinant humaan MYLPF-eiwit

Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development. Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Myosin regulatory light chain 2 skeletal muscle isoform, Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2, MYL11, Fast skeletal myosin light chain 2, MLC2B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

21.2 kDa (189aa) confirmed by MALDI-TOF

MYLPF, Myosin regulatory light chain 2 skeletal muscle isoform, Myosin regulatory light chain 2, skeletal muscle isoform, MRLC2, MYL11, Fast skeletal myosin light chain 2, MLC2B, ATGP1097-10 µg, ATGP1097-20 µg, ATGP1097-50 µg, ATGP1097-100 µg, ATGP1097-250 µg, ATGP1097-500 µg, ATGP1097-1 mg, ATGP1097-10, ATGP1097-20, ATGP1097-50, ATGP1097-100, ATGP1097-250, ATGP1097-500, ATGP1097-1

Wang Y, et al. (2007) FASEB J. 21 (9) :2205-14. ; ; Gittings W, et al. (2011) J Muscle Res Cell Motil. 31 (5-6) :337-48.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Cytoskeleton

NP_037424

Q96A32

Human

MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE