Recombinant humaan N-Acetyl-D-Glucosamine Kinase/NAGK-eiwit

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

39.8 kDa (367aa) confirmed by MALDI-TOF

N-Acetyl-D-Glucosamine Kinase, NAGK, N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase, ATGP1474-10 µg, ATGP1474-20 µg, ATGP1474-50 µg, ATGP1474-100 µg, ATGP1474-250 µg, ATGP1474-500 µg, ATGP1474-1 mg, ATGP1474-10, ATGP1474-20, ATGP1474-50, ATGP1474-100, ATGP1474-250, ATGP1474-500, ATGP1474-1

Hinderlich S, et al. (2000) Eur J Biochem 267 (11) : 3301-8.; ; Weihofen W.A., et al. (2006) J. Mol. Biol. 364:388-399

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Metabolism

NP_060037

Q9UJ70

Human

MAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS