Recombinant humaan NAA10-eiwit

NAA10 belongs to the acetyltransferase family. NAA10 interacts NAA15, HIF-1 with the ribosome. In its binding to HIF-1, NAA10 acts as a protein acetyltransferase by regulating its stability. In many cell lines, NAA10 is downregulated in response to hypoxia. NAA10 is expressed throughout the development of the brain. Recombinant human NAA10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

N (alpha) -acetyltransferase 10 NatA catalytic subunit, N (alpha) -acetyltransferase 10, NatA catalytic subunit, ARD1, ARD1A, DXS707, MGC71248, TE2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28.6 kDa (255aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NAA, N (alpha) -acetyltransferase 10 NatA catalytic subunit, N (alpha) -acetyltransferase 10, NatA catalytic subunit, ARD1, ARD1A, DXS707, MGC71248, TE2, ATGP1159-10 µg, ATGP1159-20 µg, ATGP1159-50 µg, ATGP1159-100 µg, ATGP1159-250 µg, ATGP1159-500 µg, ATGP1159-1 mg, ATGP1159-10, ATGP1159-20, ATGP1159-50, ATGP1159-100, ATGP1159-250, ATGP1159-500, ATGP1159-1

Arnesen T., et al. (2005) Biochem. J. 386:433-443; ; Jeong J.-W., et al. (2002) Cell 111:709-720

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200 mM NaCl

Metabolism

NP_003482

P41227

Human

MNIRNARPED LMNMQHCNLL CLPENYQMKY YFYHGLSWPQ LSYIAEDENG KIVGYVLAKM EEDPDDVPHG HITSLAVKRS HRRLGLAQKL MDQASRAMIE NFNAKYVSLH VRKSNRAALH LYSNTLNFQI SEVEPKYYAD GEDAYAMKRD LTQMADELRR HLELKEKGRH VVLGAIENKV ESKGNSPPSS GEACREEKGL AAEDSGGDSK DLSEVSETTE STDVKDSSEA SDSAS