Recombinant humaan NABP1-eiwit

Nucleic acid binding protein 1, also known as NABP1, is component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, the protein acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. This protein is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. Recombinant human NABP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleic acid binding protein 1, OBFC2A, SOSS-B2, SSB2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.8 kDa (227aa) confirmed by MALDI-TOF

NABP1, Nucleic acid binding protein 1, OBFC2A, SOSS-B2, SSB2, ATGP2353-10 µg, ATGP2353-20 µg, ATGP2353-50 µg, ATGP2353-100 µg, ATGP2353-250 µg, ATGP2353-500 µg, ATGP2353-1 mg, ATGP2353-10, ATGP2353-20, ATGP2353-50, ATGP2353-100, ATGP2353-250, ATGP2353-500, ATGP2353-1

Li Y., et al. (2009) J. Biol. Chem. 284:23525-23531; ; Huang J., et al. (2009) Mol. Cell. 35:384-393

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Epigenomics (Transcription & Translation)

NP_001026886

Q96AH0

Human

MNRVNDP LIFIRDIKPG LKNLNVVFIV LEIGRVTKTK DGHEVRSCKV ADKTGSITIS VWDEIGGLIQ PGDIIRLTRG YASMWKGCLT LYTGRGGELQ KIGEFCMVYS EVPNFSEPNP DYRGQQNKGA QSEQKNNSMN SNMGTGTFGP VGNGVHTGPE SREHQFSHAG RSNGRGLINP QLQGTASNQT VMTTISNGRD PRRAFKR