Recombinant humaan Nanog-eiwit

NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.6 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ATGP1124-10 µg, ATGP1124-20 µg, ATGP1124-50 µg, ATGP1124-100 µg, ATGP1124-250 µg, ATGP1124-500 µg, ATGP1124-1 mg, ATGP1124-010, ATGP1124-020, ATGP1124-050, ATGP1124-100, ATGP1124-250, ATGP1124-500, ATGP1124-01M

Clark A T., et al. (2004) Stem Cells. 22:169-179.; ; Chambers I., et al. (2003) Cell Res. 13:499-502.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_079141

Q9H9S0

Human

MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ