Recombinant humaan NANS-eiwit

NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins) -like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sialic acid synthase, SAS, N acetylneuraminate synthase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

42.4 kDa (379aa) confirmed by MALDI-TOF

NANS, Sialic acid synthase, SAS, N acetylneuraminate synthase, ATGP0784-10 µg, ATGP0784-20 µg, ATGP0784-50 µg, ATGP0784-100 µg, ATGP0784-250 µg, ATGP0784-500 µg, ATGP0784-1 mg, ATGP0784-10, ATGP0784-20, ATGP0784-50, ATGP0784-100, ATGP0784-250, ATGP0784-500, ATGP0784-1

Milstein O., et al. (2008) J Biol Chem. 283 (49) :34414-22.; ; Aldape MJ., et al. (2007) J Infect Dis. 195 (12) :1838-45.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Metabolism

NP_061819

Q9NR45

Human

MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS