Recombinant humaan NAP1L4-eiwit

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Recombinant human NAP1L4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nucleosome assembly protein 1-like 4, hNAP2, NAP1L4b, NAP2, NAP2L

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.2 kDa (398aa) confirmed by MALDI-TOF

NAP1L4, Nucleosome assembly protein 1-like 4, hNAP2, NAP1L4b, NAP2, NAP2L, ATGP2317-10 µg, ATGP2317-20 µg, ATGP2317-50 µg, ATGP2317-100 µg, ATGP2317-250 µg, ATGP2317-500 µg, ATGP2317-1 mg, ATGP2317-10, ATGP2317-20, ATGP2317-50, ATGP2317-100, ATGP2317-250, ATGP2317-500, ATGP2317-1

Olsen J.V., et al. (2006) Cell. 127:635-648; ; Nousiainen M., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:5391-5396;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_005960

Q99733

Human

MADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV