Recombinant humaan NAPA-eiwit

NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

N-ethylmaleimide-sensitive factor attachment protein alpha, NSF attachment protein alpha, SNAPA, SNAP-alpha

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.3 kDa (315aa) confirmed by MALDI-TOF

SNAP-alpha, SNAPA, SNAP alpha, NSF attachment protein alpha, N-ethylmaleimide-sensitive factor attachment protein alpha, N-ethylmaleimide sensitive factor attachment protein alpha, N ethylmaleimide sensitive factor attachment protein alpha, ATGP0708-10 µg, ATGP0708-20 µg, ATGP0708-50 µg, ATGP0708-100 µg, ATGP0708-250 µg, ATGP0708-500 µg, ATGP0708-1 mg, ATGP0708-010, ATGP0708-020, ATGP0708-050, ATGP0708-100, ATGP0708-250, ATGP0708-500, ATGP0708-01M

Thummel CS., et al. (2010) Dev Dyn. 239 (3) :954-64; ; Lee JS., et al (2002) J Basic Microbiol. 42 (4) :238-45.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Neuroscience

NP_003818

P54920

Human

MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR