Recombinant humaan NAPG-eiwit

NAPG, also known as gamma-SNAP, is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding. Recombinant human NAPG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Gamma-soluble NSF attachment protein, GAMMA-SNAP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.3 kDa (336aa) confirmed by MALDI-TOF

NAPG, Gamma-soluble NSF attachment protein, GAMMA-SNAP, ATGP1228-10 µg, ATGP1228-20 µg, ATGP1228-50 µg, ATGP1228-100 µg, ATGP1228-250 µg, ATGP1228-500 µg, ATGP1228-1 mg, ATGP1228-10, ATGP1228-20, ATGP1228-50, ATGP1228-100, ATGP1228-250, ATGP1228-500, ATGP1228-1

Bennett M.K. et al. (1993) Cell 74: 863-873.; ; McMahon H.T. et al. (1995) J. Biol. Chem. 270: 2213-2217.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Neuroscience

NP_003817.1

Q99747

Human

MAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC