Recombinant humaan NCAALD-eiwit

Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neurocalcin-delta, MGC33870, MGC74858, Neurocalcin delta

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.4 kDa (213aa) confirmed by MALDI-TOF

NCALD, Neurocalcin-delta, MGC33870, MGC74858, Neurocalcin delta, ATGP0895-10 µg, ATGP0895-20 µg, ATGP0895-50 µg, ATGP0895-100 µg, ATGP0895-250 µg, ATGP0895-500 µg, ATGP0895-1 mg, ATGP0895-10, ATGP0895-20, ATGP0895-50, ATGP0895-100, ATGP0895-250, ATGP0895-500, ATGP0895-1

Burqoyne RD., et al. (2007) Nat Rev Neurosci. 8 (3) :182-93.; ; Burqoyne RD., et al. (2004) Trends Neurosci. 27 (4) : 203-9

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 100 mM NaCl

Neuroscience

NP_001035720

P61601

Human

MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF