Recombinant humaan NCBP2-eiwit

NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Recombinant human NCBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nuclear cap-binding protein subunit 2, CBC2, CBP20, NIP1, PIG55

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

20.1 kDa (176aa) confirmed by MALDI-TOF

NCBP2, Nuclear cap-binding protein subunit 2, CBC2, CBP20, NIP1, PIG55, ATGP0747-10 µg, ATGP0747-20 µg, ATGP0747-50 µg, ATGP0747-100 µg, ATGP0747-250 µg, ATGP0747-500 µg, ATGP0747-1 mg, ATGP0747-10, ATGP0747-20, ATGP0747-50, ATGP0747-100, ATGP0747-250, ATGP0747-500, ATGP0747-1

Izaurralde E., et al. (1994) Cell. 78: 657-668.; ; Strasser A., et al. ((2005) EMBO J 24 (13) :2235-43.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Epigenomics (Transcription & Translation)

NP_031388.2

P52298

Human

MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ