Recombinant humaan NCE2/UBE2F eiwit

UBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NEDD8-conjugating enzyme uBE2F, NCE2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.7 kDa (209aa) confirmed by MALDI-TOF

UBE2N, Ubc13, NEDD8-conjugating enzyme uBE2F, NCE2, ATGP1607-10 µg, ATGP1607-20 µg, ATGP1607-50 µg, ATGP1607-100 µg, ATGP1607-250 µg, ATGP1607-500 µg, ATGP1607-1 mg, ATGP1607-10, ATGP1607-20, ATGP1607-50, ATGP1607-100, ATGP1607-250, ATGP1607-500, ATGP1607-1

Huang D.T., et al. (2009) Mol. Cell 33:483-495; ; Nandi, D. et al. (2006) Journal of biosciences 31 (1) : 137-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 200 mM NaCl

Ubiquitination

NP_542409

Q969M7

Human

MLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR