Recombinant humaan NCEH1-eiwit

Neutral cholesterol ester hydrolase 1, also known as NCEH1, hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. It may be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also NCEH1 is involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. This protein may contribute to cancer pathogenesis by promoting tumor cell migration. Recombinant human NCEH1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Neutral cholesterol ester hydrolase 1, AADACL1, NCEH

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.6 kDa (298aa)

NCEH1, Neutral cholesterol ester hydrolase 1, AADACL1, NCEH, ATGP2002-10 µg, ATGP2002-20 µg, ATGP2002-50 µg, ATGP2002-100 µg, ATGP2002-250 µg, ATGP2002-500 µg, ATGP2002-1 mg, ATGP2002-10, ATGP2002-20, ATGP2002-50, ATGP2002-100, ATGP2002-250, ATGP2002-500, ATGP2002-1

Chiang K.P., et al. (2006) Chem. Biol. 13:1041-1050; ; Okazaki H., et al. (2008) J. Biol. Chem. 283:33357-33364;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_001139750

Q6PIU2

Human

MAEELNA VIVSIEYRLV PKVYFPEQIH DVVRATKYFL KPEVLQKYMV DPGRICISGD SAGGNLAAAL GQQFTQDASL KNKLKLQALI YPVLQALDFN TPSYQQNVNT PILPRYVMVK YWVDYFKGNY DFVQAMIVNN HTSLDVEEAA AVRARLNWTS LLPASFTKNY KPVVQTTGNA RIVQELPQLL DARSAPLIAD QAVLQLLPKT YILTCEHDVL RDDGIMYAKR LESAGVEVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY IKWLDQNL