Recombinant humaan NCF4-eiwit

NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system. Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.1 kDa (359aa) confirmed by MALDI-TOF

NCF4, Neutrophil cytosol factor 4, NCF, P40PHOX, SH3PXD4, ATGP1769-10 µg, ATGP1769-20 µg, ATGP1769-50 µg, ATGP1769-100 µg, ATGP1769-250 µg, ATGP1769-500 µg, ATGP1769-1 mg, ATGP1769-10, ATGP1769-20, ATGP1769-50, ATGP1769-100, ATGP1769-250, ATGP1769-500, ATGP1769-1

Eglinton, T.W., et al. (2012) Am. J. Gastroenterol. 107 (4), 589-596

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_000622

Q15080

Human

MAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP