Recombinant humaan NCK2-eiwit

NCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptiona splice variants, encoding different isoforms, have been characterized. Recombinant human NCK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NCK adaptor protein 2, GRB4, NCKbeta

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

45.3 kDa (403aa) confirmed by MALDI-TOF

NCK2, NCK adaptor protein 2, GRB4, NCKbeta, ATGP2235-10 µg, ATGP2235-20 µg, ATGP2235-50 µg, ATGP2235-100 µg, ATGP2235-250 µg, ATGP2235-500 µg, ATGP2235-1 mg, ATGP2235-10, ATGP2235-20, ATGP2235-50, ATGP2235-100, ATGP2235-250, ATGP2235-500, ATGP2235-1

Braverman L.E., et al. (1999) J. Biol. Chem. 274:5542-5549; ; Latreille M., et al. (2006) J. Biol. Chem. 281:26633-26644

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Cancer

NP_003572

O43639

Human

MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ