Recombinant humaan NCM-1/CD56-eiwit

NCAM-1, also known as CD56, is a member of the immunoglobulin superfamily. It specifically binds to neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. This protein is preferentially expressed in NK cells and a subset of T lymphocytes that mediate MHC-unrestricted cell-mediated cytotoxicity. High expression of NCAM-1/CD56 differentiates NK cells as having an activated phenotype. CD56 (+high) NK cells mediate heightened effector functions in response to IL-12. During hematopoiesis, NCAM-1/CD56 is the prototypic marker of NK cells, also present on subset of CD4+ T cells and CD8+ T cells. In cell adhesion, it contributes to cell-cell adhesion or cell-matrix adhesion during embryonic development. In anatomic pathology, pathologists make use of it immunohistochemistry to recognize certain tumors. Recombinant human NCAM-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Neural cell adhesion molecule 1, Neural cell adhesion molecule 1 isoform3, N-CAM-1, NCAM-1, NCAM, NCAM1, CD56 antigen, CD56, MSK39, NCAM

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

65.7kDa (593aa)

, ATGP3991-10 µg, ATGP3991-20 µg, ATGP3991-50 µg, ATGP3991-100 µg, ATGP3991-250 µg, ATGP3991-500 µg, ATGP3991-1 mg, ATGP3991-10, ATGP3991-20, ATGP3991-50, ATGP3991-100, ATGP3991-250, ATGP3991-500, ATGP3991-1

Van Acker HH, et al. (2017) . Frontiers in Immunology. 8:892.; ; Sanchez-Heras, E. et al. (2006) J Biol Chem. 281:35208-35216.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_001070150

P13591

Human

Ala

LQVDIVP SQGEISVGES KFFLCQVAGD AKDKDISWFS PNGEKLTPNQ QRISVVWNDD SSSTLTIYNA NIDDAGIYKC VVTGEDGSES EATVNVKIFQ KLMFKNAPTP QEFREGEDAV IVCDVVSSLP PTIIWKHKGR DVILKKDVRF IVLSNNYLQI RGIKKTDEGT YRCEGRILAR GEINFKDIQV IVNVPPTIQA RQNIVNATAN LGQSVTLVCD AEGFPEPTMS WTKDGEQIEQ EEDDEKYIFS DDSSQLTIKK VDKNDEAEYI CIAENKAGEQ DATIHLKVFA KPKITYVENQ TAMELEEQVT LTCEASGDPI PSITWRTSTR NISSEEKTLD GHMVVRSHAR VSSLTLKSIQ YTDAGEYICT ASNTIGQDSQ SMYLEVQYAP KLQGPVAVYT WEGNQVNITC EVFAYPSATI SWFRDGQLLP SSNYSNIKIY NTPSASYLEV TPDSENDFGN YNCTAVNRIG QESLEFILVQ ADTPSSPSID QVEPYSSTAQ VQFDEPEATG GVPILKYKAE WRAVGEEVWH SKWYDAKEAS MEGIVTIVGL KPETTYAVRL AALNGKGLGE ISAASEFKTQ PVHSPPP