Recombinant humaan NCS1-eiwit

Neuronal calcium sensor 1, also known as NCS1, is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. Recombinant human NCS1 fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

22.9 kDa (198aa) confirmed by MALDI-TOF

NCS1, Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1., ATGP0526-10 µg, ATGP0526-20 µg, ATGP0526-50 µg, ATGP0526-100 µg, ATGP0526-250 µg, ATGP0526-500 µg, ATGP0526-1 mg, ATGP0526-10, ATGP0526-20, ATGP0526-50, ATGP0526-100, ATGP0526-250, ATGP0526-500, ATGP0526-1

Cox J A., et al. (1994) J Biol Chem. 269:32807-32813.; ; McFerran., et al. (1998) J Biol Chem. 273:22768-22772.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Neuroscience

NP_055101

P62166

Human

MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH