Recombinant humaan NCS1-eiwit

NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. It is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human NCS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neuronal calcium sensor 1 isoform 1, FLuP, FREQ

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.3. kDa (213aa) confirmed by MALDI-TOF

NCS1, Neuronal calcium sensor 1 isoform 1, FLuP, FREQ, ATGP2807-10 µg, ATGP2807-20 µg, ATGP2807-50 µg, ATGP2807-100 µg, ATGP2807-250 µg, ATGP2807-500 µg, ATGP2807-1 mg, ATGP2807-10, ATGP2807-20, ATGP2807-50, ATGP2807-100, ATGP2807-250, ATGP2807-500, ATGP2807-1

Nakamura T.Y., et al (2001) . Proc. Natl. Acad. Sci. u.S.A. 98:12808-12813; ; Bahi N., et al (2003) . Hum. Mol. Genet. 12:1415-1425

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Neuroscience

NP_055101

P62166

Human

MGKSNSK LKPEVVEELT RKTYFTEKEV QQWYKGFIKD CPSGQLDAAG FQKIYKQFFP FGDPTKFATF VFNVFDENKD GRIEFSEFIQ ALSVTSRGTL DEKLRWAFKL YDLDNDGYIT RNEMLDIVDA IYQMVGNTVE LPEEENTPEK RVDRIFAMMD KNADGKLTLQ EFQEGSKADP SIVQALSLYD GLV