Recombinant humaan NDE1-eiwit

NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans)

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.9 kDa (355aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NDE1, Nuclear distribution protein nudE homolog 1, FLJ20101, HOM-TES-87, NuDE, NuDE, nudE nuclear distribution gene E homolog 1 (A. nidulans), ATGP1027-10 µg, ATGP1027-20 µg, ATGP1027-50 µg, ATGP1027-100 µg, ATGP1027-250 µg, ATGP1027-500 µg, ATGP1027-1 mg, ATGP1027-10, ATGP1027-20, ATGP1027-50, ATGP1027-100, ATGP1027-250, ATGP1027-500, ATGP1027-1

Yan X., et al. (2003) Mol Cell Biol. 23 (4) :1239-50. ; ; Kitagawa M., et al. (2000) FEBS Lett. 479 (1-2) :57-62.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl.

Cell Cycle

NP_001137451

Q9NXR1

Human

MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC