Recombinant humaan NDFIP1-eiwit

NDFIP1 belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. Recombinant human NDFIP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

NEDD4 family-interacting protein 1, N4WBP5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.8 kDa (139aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NDFIP1, NEDD4 family-interacting protein 1, N4WBP5, ATGP2468-10 µg, ATGP2468-20 µg, ATGP2468-50 µg, ATGP2468-100 µg, ATGP2468-250 µg, ATGP2468-500 µg, ATGP2468-1 mg, ATGP2468-10, ATGP2468-20, ATGP2468-50, ATGP2468-100, ATGP2468-250, ATGP2468-500, ATGP2468-1

Wang Y. et al. (2012) J Immunol. 189:5304-5313; ; Howitt J. et al. (2012) J Cell Biol. 196:29-36.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Ubiquitination

NP_085048

Q9BT67

Human

MGSSHHHHHH SSGLVPRGSH MGSMALALAA LAAVEPACGS RYQQLQNEEE SGEPEQAAGD APPPYSSISA ESAAYFDYKD ESGFPKPPSY NVATTLPSYD EAERTKAEAT IPLVPGRDED FVGRDDFDDA DQLRIGNDG