Recombinant humaan NDUFA2-eiwit

NDuFA2 is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox. Mutations in this protein are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Recombinant human NDuFA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2, Complex I-B8, CI-B8, NADH-ubiquinone oxidoreductase B8 subunit, NADH:ubiquinone oxidoreductase subunit A2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

13.3 kDa (122aa) confirmed by MALDI-TOF

NDUFA2, NADH dehydrogenase 1 alpha subcomplex, B8, CD14, CIB8, ATGP1770-10 µg, ATGP1770-20 µg, ATGP1770-50 µg, ATGP1770-100 µg, ATGP1770-250 µg, ATGP1770-500 µg, ATGP1770-1 mg, ATGP1770-10, ATGP1770-20, ATGP1770-50, ATGP1770-100, ATGP1770-250, ATGP1770-500, ATGP1770-1

Murray, J., et al. (2003) J. Biol. Chem. 278 (16), 13619-13622; ; Loeffen, J.L.., et al. (1998) Biochem. Biophys. Res. Commun. 253 (2), 415-422.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_002479

O43678

Human

MAAAAAS RGVGAKLGLR EIRIHLCQRS PGSQGVRDFI EKRYVELKKA NPDLPILIRE CSDVQPKLWA RYAFGQETNV PLNNFSADQV TRALENVLSG KA