Recombinant humaan NDUFA5-eiwit

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDuFA5, belongs to the complex I NDuFA5 subunit family. The human NDuFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDuFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. Recombinant human NDuFA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

NADH:ubiquinone oxidoreductase subunit A5, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, Complex I subunit B13, Complex I-13kD-B, CI-13kD-B, NADH-ubiquinone oxidoreductase 13 kDa-B subunit, Complex I 13kDa subunit B, Ubiquinone reductase, Type I dehydrogenase, B13, NUFM, UQOR13, CI-13kB

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.8 kDa (139aa) confirmed by MALDI-TOF

NDUFA5, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, B13, CI-13kB, CI-13KD-B, NuFM, uQOR13, ATGP1762-10 µg, ATGP1762-20 µg, ATGP1762-50 µg, ATGP1762-100 µg, ATGP1762-250 µg, ATGP1762-500 µg, ATGP1762-1 mg, ATGP1762-10, ATGP1762-20, ATGP1762-50, ATGP1762-100, ATGP1762-250, ATGP1762-500, ATGP1762-1

Murray J., et al. (2003) J. Biol. Chem. 278:13619-13622; ; Choudhary C., et al. (2009) Science. 325:834-840

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_004991

Q16718

Human

MAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI