Recombinant humaan NDUFAF1-eiwit

NDuFAF1 is a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The protein is required for assembly of complex I, and mutations in this protein are a cause of mitochondrial complex I deficiency. Recombinant human NDuFAF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH:ubiquinone oxidoreductase complex assembly factor 1, Complex I intermediate-associated protein 30 mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, CIA30, CGI-65

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37 kDa (326aa) confirmed by MALDI-TOF

NDUFAF1, Complex I intermediate-associated protein 30, CGI-65; CGI65; CIA30, ATGP1775-10 µg, ATGP1775-20 µg, ATGP1775-50 µg, ATGP1775-100 µg, ATGP1775-250 µg, ATGP1775-500 µg, ATGP1775-1 mg, ATGP1775-10, ATGP1775-20, ATGP1775-50, ATGP1775-100, ATGP1775-250, ATGP1775-500, ATGP1775-1

Vogel RO., et al. (2005) . FEBS J. 272 (20) :5317-26.; ; Janssen R., et al. (2002) . Hum Genet. 110 (3) :264-70.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT, 2mM EDTA

Metabolism

NP_057097

Q9Y375

Human

MGSSHHHHHH SSGLVPRGSH MGSYPFLGIR FAEYSSSLQK PVASPGKASS QRKTEGDLQG DHQKEVALDI TSSEEKPDVS FDKAIRDEAI YHFRLLKDEI VDHWRGPEGH PLHEVLLEQA KVVWQFRGKE DLDKWTVTSD KTIGGRSEVF LKMGKNNQSA LLYGTLSSEA PQDGESTRSG YCAMISRIPR GAFERKMSYD WSQFNTLYLR VRGDGRPWMV NIKEDTDFFQ RTNQMYSYFM FTRGGPYWQE VKIPFSKFFF SNRGRIRDVQ HELPLDKISS IGFTLADKVD GPFFLEIDFI GVFTDPAHTE EFAYENSPEL NPRLFK