Recombinant humaan NDUFAF4-eiwit

DH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, also known as NDuFAF4, is involved in the assembly of mitochondrial NADH: ubiquinone oxidoreductase complex (complex I) . This protein may be involved in cell proliferation and survival of hormone-dependent tumor cells. This protein may be a regulator of breast tumor cell invasion. Recombinant human NDuFAF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, bA22L21.1, C6orf66, HRPAP20, HSPC125, My013, NADH:ubiquinone oxidoreductase complex assembly factor 4, Hormone-regulated proliferation-associated protein of 20 kDa

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

22.7 kDa (198aa) confirmed by MALDI-TOF

NDUFAF4, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, bA22L21.1, C6orf66, HRPAP20, HSPC125, My013, ATGP1767-10 µg, ATGP1767-20 µg, ATGP1767-50 µg, ATGP1767-100 µg, ATGP1767-250 µg, ATGP1767-500 µg, ATGP1767-1 mg, ATGP1767-10, ATGP1767-20, ATGP1767-50, ATGP1767-100, ATGP1767-250, ATGP1767-500, ATGP1767-1

Karp C.M., et al. (2007) Oncogene. 26:1780-1788; ; Saada A., et al. (2008) Am. J. Hum. Genet. 82:32-38

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_054884

Q9P032

Human

MGSSHHHHHH SSGLVPRGSH MGSMGALVIR GIRNFNLENR AEREISKMKP SVAPRHPSTN SLLREQISLY PEVKGEIARK DEKLLSFLKD VYVDSKDPVS SLQVKAAETC QEPKEFRLPK DHHFDMINIK SIPKGKISIV EALTLLNNHK LFPETWTAEK IMQEYQLEQK DVNSLLKYFV TFEVEIFPPE DKKAIRSK