Recombinant humaan NDUFS3-eiwit

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, also known as NDuFS3, belongs to the complex I 30 kDa subunit family. The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. Recombinant human NDuFS3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, CI-30, NADH:ubiquinone oxidoreductase core subunit S3, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase (ubiquinone) Fe-S protein 3 30kDa, NADH-coenzyme Q reductase

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.7 kDa (249aa) confirmed by MALDI-TOF

NDUFS3, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, CI-30, ATGP1785-10 µg, ATGP1785-20 µg, ATGP1785-50 µg, ATGP1785-100 µg, ATGP1785-250 µg, ATGP1785-500 µg, ATGP1785-1 mg, ATGP1785-10, ATGP1785-20, ATGP1785-50, ATGP1785-100, ATGP1785-250, ATGP1785-500, ATGP1785-1

Murray J., et al. (2003) J. Biol. Chem. 278:13619-13622; ; Saada A., et al. (2009) Am. J. Hum. Genet. 84:718-727;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_004542

O75489

Human

ESAGADTRP TVRPRNDVAH KQLSAFGEYV AEILPKYVQQ VQVSCFNELE VCIHPDGVIP VLTFLRDHTN AQFKSLVDLT AVDVPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPIES AVSVFKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG HPFRKDFPLS GYVELRYDDE VKRVVAEPVE LAQEFRKFDL NSPWEAFPVY RQPPESLKLE AGDKKPDAK