Recombinant humaan NDUFS6-eiwit

NDuFS6 is a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit is one of seven subunits in the iron-sulfur protein fraction. Mutations cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders. Recombinant human NDuFS6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, CI-13Ka, CI-13kD-A, CI13KDA, NADH:ubiquinone oxidoreductase subunit S6, NADH-ubiquinone oxidoreductase 13 kDa-A subunit, NADH dehydrogenase (ubiquinone) Fe-S protein 6, Complex I 13kDa subunit A, Complex I-13kD-A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

13.2 kDa (120aa) confirmed by MALDI-TOF

NDUFS6, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, CI-13Ka, CI-13kD-A, CI13KDA, ATGP2206-10 µg, ATGP2206-20 µg, ATGP2206-50 µg, ATGP2206-100 µg, ATGP2206-250 µg, ATGP2206-500 µg, ATGP2206-1 mg, ATGP2206-10, ATGP2206-20, ATGP2206-50, ATGP2206-100, ATGP2206-250, ATGP2206-500, ATGP2206-1

Hendrickson, S.L., et al. (2010) PLoS ONE 5 (9), E12862; ; Saito, A., et al. (2009) J. Hum. Genet. 54 (6), 317-323

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Metabolism

NP_004544

O75380

Human

MGSSHHHHHH SSGLVPRGSH MGSFGVRVSP TGEKVTHTGQ VYDDKDYRRI RFVGRQKEVN ENFAIDLIAE QPVSEVETRV IACDGGGGAL GHPKVYINLD KETKTGTCGY CGLQFRQHHH