Recombinant humaan NDUFV2-eiwit

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. NDUFV2 is the 24 kDa subunit of complex I, and is involved in electron transfer. Mutations in this gene are implicated in Parkinson's disease, bipolar disorder, schizophrenia, and have been found in one case of early onset hypertrophic cardiomyopathy and encephalopathy. A non-transcribed pseudogene of this locus is found on chromosome 19. Recombinant human NDUFV2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH dehydrogenase [ubiquinone] flavoprotein 2, CI-24k, NADH:ubiquinone oxidoreductase core subunit V2, Complex I 24kDa subunit

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.1 kDa (240aa) confirmed by MALDI-TOF

NDuFV2, NADH dehydrogenase [ubiquinone] flavoprotein 2, CI-24k, ATGP2313-10 µg, ATGP2313-20 µg, ATGP2313-50 µg, ATGP2313-100 µg, ATGP2313-250 µg, ATGP2313-500 µg, ATGP2313-1 mg, ATGP2313-10, ATGP2313-20, ATGP2313-50, ATGP2313-100, ATGP2313-250, ATGP2313-500, ATGP2313-1

Murray J., et al. (2003) J. Biol. Chem. 278:13619-13622; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_066552

P19404

Human

GAGGALF VHRDTPENNP DTPFDFTPEN YKRIEAIVKN YPEGHKAAAV LPVLDLAQRQ NGWLPISAMN KVAEVLQVPP MRVYEVATFY TMYNRKPVGK YHIQVCTTTP CMLRNSDSIL EAIQKKLGIK VGETTPDKLF TLIEVECLGA CVNAPMVQIN DNYYEDLTAK DIEEIIDELK AGKIPKPGPR SGRFSCEPAG GLTSLTEPPK GPGFGVQAGL