Recombinant humaan NDUFV3-eiwit

NDuFV3 is one of at least forty-one subunits that make up the NADH-ubiquinone oxidoreductase complex. This complex is part of the mitochondrial respiratory chain and serves to catalyze the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. The protein is one of three proteins found in the flavoprotein fraction of the complex. The specific function of the encoded protein is unknown. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human NDuFV3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NADH:ubiquinone oxidoreductase subunit V3, NADH dehydrogenase [ubiquinone] flavoprotein 3 mitochondrial, Complex I-9kD, CI-9kD, complex I 10kDa subunit, CI-10k, NADH-ubiquinone oxidoreductase 9 kDa subunit, Renal carcinoma antigen NY-REN-4

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

10.8 kDa (97aa) confirmed by MALDI-TOF

NDuFV3, NADH dehydrogenase (ubiquinone) flavoprotein 3, CI-10k, CI-9KD, ATGP2345-10 µg, ATGP2345-20 µg, ATGP2345-50 µg, ATGP2345-100 µg, ATGP2345-250 µg, ATGP2345-500 µg, ATGP2345-1 mg, ATGP2345-10, ATGP2345-20, ATGP2345-50, ATGP2345-100, ATGP2345-250, ATGP2345-500, ATGP2345-1

Hendrickson, S.L., et al. (2010) PLoS ONE 5 (9), E12862; ; Saito, A., et al. (2009) J. Hum. Genet. 54 (6), 317-323;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Metabolism

NP_001001503

P56181

Human

SAESGKS EKGQPQNSKK QSPPKKPAPV PAEPFDNTTY KNLQHHDYST YTFLDLNLEL SKFRMPQPSS GRESPRH