Recombinant humaan NECAP2-eiwit

NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s alpha-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis. Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NECAP endocytosis associated 2, FLJ10420

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30.5 kDa (283aa) confirmed by MALDI-TOF

NECAP2, NECAP endocytosis associated 2, FLJ10420, ATGP0973-10 µg, ATGP0973-20 µg, ATGP0973-50 µg, ATGP0973-100 µg, ATGP0973-250 µg, ATGP0973-500 µg, ATGP0973-1 mg, ATGP0973-10, ATGP0973-20, ATGP0973-50, ATGP0973-100, ATGP0973-250, ATGP0973-500, ATGP0973-1

Miller GJ., et al. (2003) Nat Struct Biol. 10 (8) :599-606. ; ; Mattera R., et al. (2004) J Biol Chem. 279 (9) :8018-28.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Signal Transduction

NP_060560

Q9NVZ3

Human

MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF