Recombinant humaan Nectine-1-eiwit

Nectin-1, also known as PVRL1, is a poliovirus receptor- related 1 protein which belongs to the nectin family. This protein promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. It is functions as an entry receptor for herpes simplex virus and pseudorabies virus. Also, PVRL1 has some neurite outgrowth-promoting activity. Recombinant human PVRL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

PVRL1, CD111, CLPED1, ED4, HIgR, HVIS, HVEC, Nectin-1, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

37.3 kDa (334aa)

Nectin1, PVRL1, CD111, CLPED1, ED4, HIgR, HVIS, HVEC, Nectin-1, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12, ATGP3283-10 µg, ATGP3283-20 µg, ATGP3283-50 µg, ATGP3283-100 µg, ATGP3283-250 µg, ATGP3283-500 µg, ATGP3283-1 mg, ATGP3283-10, ATGP3283-20, ATGP3283-50, ATGP3283-100, ATGP3283-250, ATGP3283-500, ATGP3283-1

Bojesen KB., et al. (2012) J. Biol. Chem. 287 (44) :37420-37433.; ; Cheng HQ., et al. (2012) DNA Cell Biol. 31 (7) :1321-1327.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_002846

Q15223

Human

ADPQVVQVND SMYGFIGTDV VLHCSFANPL PSVKITQVTW QKSTNGSKQN VAIYNPSMGV SVLAPYRERV EFLRPSFTDG TIRLSRLELE DEGVYICEFA TFPTGNRESQ LNLTVMAKPT NWIEGTQAVL RAKKGQDDKV LVATCTSANG KPPSVVSWET RLKGEAEYQE IRNPNGTVTV ISRYRLVPSR EAHQQSLACI VNYHMDRFKE SLTLNVQYEP EVTIEGFDGN WYLQRMDVKL TCKADANPPA TEYHWTTLNG SLPKGVEAQN RTLFFKGPIN YSLAGTYICE ATNPIGTRSG QVEVNITEFP YTPSPPEHGR RAGPVPTAHH HHHH