Recombinant humaan NEDP1/SENP8-eiwit

SENP8 is a cysteine protease that is a member of the sentrin-specific protease family. This protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8 (NEDD8) . Recombinant human SENP8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SuMO/sentrin specific peptidase family member 8, DEN1, NEDP1, PRSC2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

26.2 kDa (232aa) confirmed by MALDI-TOF

NEDP1, SENP8, SuMO/sentrin specific peptidase family member 8, DEN1, NEDP1, PRSC2, ATGP1112-10 µg, ATGP1112-20 µg, ATGP1112-50 µg, ATGP1112-100 µg, ATGP1112-250 µg, ATGP1112-500 µg, ATGP1112-1 mg, ATGP1112-10, ATGP1112-20, ATGP1112-50, ATGP1112-100, ATGP1112-250, ATGP1112-500, ATGP1112-1

Reverter D., et al. (2004) Atructure. 12 (8) :1519-31.; ; Shen LN., et al. (2005) EMBO J. 24 (7) :1341-51.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Ubiquitination

NP_001159812

Q96LD8

Human

MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK