Recombinant humaan NEIL1-eiwit

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Endonuclease 8-like 1, FLJ22402, FPG1, hFPG1, NEI1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

45.8 kDa (410aa) confirmed by MALDI-TOF

NEIL1, Endonuclease 8-like 1, FLJ22402, FPG1, hFPG1, NEI1, ATGP1347-10 µg, ATGP1347-20 µg, ATGP1347-50 µg, ATGP1347-100 µg, ATGP1347-250 µg, ATGP1347-500 µg, ATGP1347-1 mg, ATGP1347-10, ATGP1347-20, ATGP1347-50, ATGP1347-100, ATGP1347-250, ATGP1347-500, ATGP1347-1

Hazra T.K. et al. (2002) J. Biol.Chem. 277: 30417-30420.; ; Dou H. et al. (2003) J. Biol.Chem. 278: 49679-49684.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Enzymes & Proteases

NP_078884.2

Q96FI4

Human

MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS