Recombinant humaan NEIL2-eiwit

Endonuclease 8-like 2, also known as NEIL2, belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. It has negligible or undetectable activity with 8-oxoguanine, thymine glycol, 2-hydroxyadenine, hypoxanthine, and xanthine. Recombinant human NEIL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Endonuclease 8-like 2, NEH2, NEI2, Nei-like protein 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.4 kDa (356aa) confirmed by MALDI-TOF

NEIL2, Endonuclease 8-like 2, NEH2, NEI2, Nei-like protein 2, ATGP1526-10 µg, ATGP1526-20 µg, ATGP1526-50 µg, ATGP1526-100 µg, ATGP1526-250 µg, ATGP1526-500 µg, ATGP1526-1 mg, ATGP1526-10, ATGP1526-20, ATGP1526-50, ATGP1526-100, ATGP1526-250, ATGP1526-500, ATGP1526-1

Hazra TK., et al. (2002) J Biol Chem. 277 (34) :30417-20.; ; Das D., et al. (2007) J Biol Chem. 282 (39) :28474-84.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_659480

Q969S2

Human

MGSSHHHHHH SSGLVPRGSH MGSHMPEGPL VRKFHHLVSP FVGQQVVKTG GSSKKLQPAS LQSLWLQDTQ VHGKKLFLRF DLDEEMGPPG SSPTPEPPQK EVQKEGAADP KQVGEPSGQK TLDGSSRSAE LVPQGEDDSE YLERDAPAGD AGRWLRVSFG LFGSVWVNDF SRAKKANKRG DWRDPSPRLV LHFGGGGFLA FYNCQLSWSS SPVVTPTCDI LSEKFHRGQA LEALGQAQPV CYTLLDQRYF SGLGNIIKNE ALYRAGIHPL SLGSVLSASR REVLVDHVVE FSTAWLQGKF QGRPQHTQVY QKEQCPAGHQ VMKEAFGPED GLQRLTWWCP QCQPQLSEEP EQCQFS