Recombinant humaan NEK7-eiwit

NEK7 is protein kinase which plays an important role in mitotic cell cycle progression. It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Recombinant human NEK7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Serine/threonine-protein kinase Nek7, NIMA (never in mitosis gene a) -related kinase 7

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37 kDa (326aa) confirmed by MALDI-TOF

NEK7, Serine/threonine-protein kinase Nek7, NIMA (never in mitosis gene a) -related kinase 7, ATGP1787-10 µg, ATGP1787-20 µg, ATGP1787-50 µg, ATGP1787-100 µg, ATGP1787-250 µg, ATGP1787-500 µg, ATGP1787-1 mg, ATGP1787-10, ATGP1787-20, ATGP1787-50, ATGP1787-100, ATGP1787-250, ATGP1787-500, ATGP1787-1

Yissachar N, Salem H, et al. (2006) . FEBS Lett. 580 (27) :6489-95. ; ; Kim S, Lee K, et al. (2007) . Biochem Biophys Res Commun. 360 (1) :56-62. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Cell Cycle

NP_598001

Q8TDX7

Human

MDEQSQ GMQGPPVPQF QPQKALRPDM GYNTLANFRI EKKIGRGQFS EVYRAACLLD GVPVALKKVQ IFDLMDAKAR ADCIKEIDLL KQLNHPNVIK YYASFIEDNE LNIVLELADA GDLSRMIKHF KKQKRLIPER TVWKYFVQLC SALEHMHSRR VMHRDIKPAN VFITATGVVK LGDLGLGRFF SSKTTAAHSL VGTPYYMSPE RIHENGYNFK SDIWSLGCLL YEMAALQSPF YGDKMNLYSL CKKIEQCDYP PLPSDHYSEE LRQLVNMCIN PDPEKRPDVT YVYDVAKRMH ACTASS